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The Pixelgen Proxiome Kit - Mechanistic understanding of cell function by protein interactomics

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Pixelgen Proxiome Kit v2, Immuno 155 v2

The Pixelgen Proxiome Kit v2 enables high-dimensional analysis of cell surface architecture in single human immune cells, generating readouts on protein abundance, protein clustering, and protein colocalization for 155 surface proteins. It is provided as a sample prep kit enabling direct implementation in any lab, without the need for acquiring expensive specialized equipment. The kit is available in 8-, 16-, and 32-reaction formats, supporting up to 64 samples per experiment, and can be combined with the Pixelgen Proxiome Custom Conjugation and Pixelgen Proxiome CAR T products for increased customization.

Pixelgen Proxiome Kit v2, Immuno 155 v2

8 reactions                 Part nr.: PROXIMM0028

16 reactions               Part nr.: PROXIMM00216

32 reactions               Part nr.: PROXIMM00232

Product Features

  • Research areas: hematology, CAR-T / cell therapy, immuno-oncology, autoimmunity, infectious disease, fundamental immunology, and more
  • Readout: Cell surface architecture analysis of human immune cells
  • Data output: Single cell proximity networks with associated abundance, clustering, and colocalization scores for each cell
  • Resolution: Nanoscale (approximately ~50 nm)
  • Species: Human
  • Multiplex: 155 cell surface proteins
  • Add-ons: Support for up to 2 custom antibodies using the Pixelgen Proxiome v2 Custom Conjugation Kit and up to 2 CAR-specific detection reagents through the Pixelgen Proxiome CAR T products 
  • Throughput: Up to 64 samples per experiment
  • Cell input: from 50 000 cells per sample
  • Cell output: 1000 cells per sample
  • Supported sample input: Cells in solution originating from live or frozen PBMCs, bone marrow, synovial fluid, isolated immune cells, cell lines, cells dissociated from organoids or fresh frozen tissue etc. 
  • Workflow: Standard molecular biology workflow with sequencing support for Illumina, MGI, Roche, Ultima Genomics, and AVITI platforms
  • Analysis: Pixelator software for data processing, spatial analysis, and integration with standard single-cell analysis workflows
3M9A5176

Product Sheet

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Protein List

 These 155 proteins and 3 controls were selected to enable high-dimensional mapping of immune cell surface architecture using the Proximity Network Assay.

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