Spatial cell biology
Toward a Better Understanding of Dynamic Cell Regulation. While significant cell mapping efforts have focused on the gene expression characteristics of single cells, understanding dynamic cell regulation through spatial reorganization of proteins remains largely uncharted.
Molecular Pixelation Offers Detailed Three-Dimensional Spatial Mapping of Cell Surface Proteins
Our revolutionary Molecular Pixelation technology delivers next dimension, single-cell, spatial proteomics to advance human health from basic research to drug discovery and development and diagnostics. Molecular Pixelation offers deep cellular phenotyping by revealing polarization and co-localization of proteins at high multiplex. Pixelation is enabled by cell-surface zonation using DNA barcoding, and data is then presented in a three-dimensional spatial map.
Molecular Pixelation - How it works
Molecular Pixelation uses DNA-conjugated antibodies and DNA encoded pixels to identify and spatially locate cell surface proteins.
1. Staining cells in solution with DNA-conjugated antibodies
2. Molecular Pixelation introduces zone barcodes
3. NGS library preparation
4. NGS sequencing
5. Data analysis providing 3D representation of cell surface proteins
Key Features & Benefits
3D Spatial Single Cell Proteomics
Molecular Pixelation (MPX) enables highly resolved cell surface protein maps for deep phenotyping of immune cells. MPX provides a deeper understanding of cell biology, disease causing mechanisms and drug mode of action.
Unprecedented spatial protein multiplexing
DNA tagged monoclonal antibodies ensure high multiplex analysis of cell surface proteins with validated target specificity. The barcoding approach enables a smooth transition from analog to digital data readout.
In Focus without a Microscope
Forget your Z-stacks, stitching, auto-fluorescence, fluorophore-bleed through and bleaching! Use a single tube, partitioning-free sample preparation, providing spatially resolved abundance data similar to Confocal and Flow Cytometry, always in focus and from every angle.
Leverage your existing NGS workflow
Obtain powerful single cell protein abundance, polarization and co-localization data with a simple, scalable solution available to any size cell and molecular biology laboratory.