CAR T Cells & Cell Therapy

Characterize engineered immune cells beyond expression profiling

CAR design influences not only antigen recognition, but also receptor organization, signaling, and downstream cellular function. The Proximity Network Assay enables nanoscale mapping of CAR localization, clustering, and interaction partners in single cells, supporting deeper characterization of activation states, exhaustion, persistence, and target engagement.

Applications include:

• CAR membrane organization 
• Tonic signaling and activation states 
• Exhaustion-associated remodeling
• Trogocytosis and conjugate formation

Identify interaction partners of therapeutic receptors. Here, the proxiome of the CD19 Chimeric Antigen Receptor is shown. Learn more about CAR T cell phenotyping here.

Hematology & Immuno-Oncology

Discover disease mechanisms in hematological disease

Hematological malignancies are driven by complex changes in receptor signaling. The Proximity Network Assay enables high-dimensional profiling of tumor cell surface architecture, revealing spatial protein networks associated with disease state, therapeutic response, and antibody mode-of-action.

Applications include:

• Lymphoma and leukemia mechanistic profiling 
• Therapeutic antibody characterization 
• Immune checkpoint biology 
• Tumor heterogeneity analysis 
• Spatial biomarker discovery 

  
  
  Unravel cancer-specific spatial biomarkers using protein interactomics analysis, here showcased on Burkitt’s lymphoma cells. Learn more about hematological profiling here.
  

Autoimmunity & Inflammation

Decode dysfunctional immune signaling

Autoimmune diseases emerge from dysregulated immune activation and aberrant cellular interactions. The Proximity Network Assay enables characterization of immune cell surface architecture at single-cell resolution, revealing spatial protein signatures linked to pathogenic immune states and therapeutic responses.

Applications include:

• Exhaustion and chronic signaling 
• Immune cell interactions
• Mechanisms of immune dysregulation
• Biomarker discovery in inflammatory disease

Identify disease mechanisms in autoimmune disease. Petter Brodin showed that, although being downregulated, CD21 displays increased protein clustering in SLE patients. Learn more about autoimmune cell profiling here

Fundamental & Translational Immunology

Study immune cell biology at nanoscale resolution

Protein abundance alone cannot fully explain immune cell behavior. The Proximity Network Assay enables researchers to study how proteins organize into functional membrane systems controlling signaling, migration, adhesion, and cell–cell communication.

Applications include:

• Receptor signaling biology 
• Lipid raft and membrane domain analysis 
• Cell–cell interaction studies
• Functional immune cell heterogeneity

Understand immune cell regulation at the molecular level, during activation and functional responses such as migration. Learn more here