Frequently Asked Questions

Welcome to our library of frequently asked questions!

The FAQs are grouped into categories of related topics for your convenience:

  • Click the category title to see to expand the number of questions and answers under each category
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  • In case your question is not answered here, an enquiry form is available on each of the individual FAQs

Protocol

What automatic cell counters can you recommend?

The Countess II Automated Cell Counter (ThermoFisher) has been used during validation and optimization of MPX™.

What manual cell counters can you recommend?

Hemocytometers and single-use counting hemocytometers have been used during validation and optimization of MPX™.

Are there stopping points in the protocol?

Yes, the protocol contains many stopping points to offer flexibility.

How long does it take to run the protocol?

If you intend to run the protocol consecutively from live cells to sequence-ready library, we recommend planning for 3 days. If you intend to fix live cells and then freeze for -80C storage for up to 3 months, we recommend 2 hours for fixing and freezing, and then 2 days for thawing and running the remainder of the protocol.

Can you recommend a workflow timeline to me?

A suggested workflow would be to perform fixation and AOC binding day 1, perform pixelation steps on day 2 and finally quality control and NGS on day 3.

Can I use other PCR primers than supplied with the kit?

If there is a need to use other PCR primers than the ones supplied with the kit, please contact link: support for guidance.

Can I run MPX on frozen live PBMCs as starting material?

The workflow has been validated on frozen fixed cells and we currently have not evaluated running the protocol on frozen live cells.

How many cells are needed as input?

MPX requires 500 000 – 1 000 000 live cells as input.

Panel design

Can I include personal targets in the panel?

The panel included in the kit is fixed with targets that have been validated. We do have a target wish list where we are more than happy to take your feedback, please fill it in here: link.

Can you provide full custom panels?

The product we offer at this stage contains a fixed panel, The Immunology Panel I, Human. We are open to discussing your interest in different targets for potential future panels, see the target wish list here: link.

Can I suggest targets for coming panels?

We have a target wish list where we are more than happy to take your feedback, please fill it in here: link.

What targets are included in your panel?

Please find the complete list of targets here.

How was the panel designed?

The panel was designed to involve both backbone proteins for different immune cells in but also

Assay design

What does AOC stand for?

Antibody Oligonucleotide Conjugate

What type of antibodies do you use in your assay design?

The AOC panel in MPX™ consists of monoclonal antibodies.

How have you designed the DNA oligo that you use for conjugating the antibodies?

To be updated

How do you choose your antibodies?

To be updated

Can you compare the relative abundance of two different proteins on the same cell?

To be updated

How do you validate your Abs?

The specificity of each antibody was first validated prior to conjugation using flow cytometry on PFA-fixed cells, and the specificity of each AOC was validated using MPX.

How do you validate your Antibodies?

The specificity of each antibody was first validated prior to conjugation using flow cytometry on PFA-fixed cells, and the specificity of each AOC was validated using MPX.

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