Pixelgen Technologies today launched the Pixelgen Proxiome Kit v2, a second-generation release of its Proxiome platform that expands the scale of single cell surface analysis to translational and clinical applications. The update introduces new high-throughput configurations, allowing up to 64 samples to be processed in one experiment block and the capability to add custom protein targets to the Proxiome panel.
The Proxiome platform is built to deliver deeper insight into cell surface architecture, capturing the spatial organization of proteins on the surface of single cells and uncovering what this organization reveals about cell state, disease, and treatment response. While existing tools measure protein abundance, the Proximity Network Assay, the foundational technology in the Proxiome Kit, reaches the architectural layer where proteins cluster, colocalize, and assemble into functional complexes at the nanoscale. This layer is where signaling actually operates, and until now, it has remained inaccessible at a meaningful and applicable level.
“Single cell analysis of cell surface architecture has moved beyond pilot experiments,” said Pixelgen Chief Technology Officer Filip Karlsson. “Our Proxiome Kit v2 not only reveals how proteins are organized at the nanoscale across thousands of single cells, but it now brings Cell Surface Architecture analysis to the scale that translational work actually demands, allowing the study of larger cohorts, time courses, and multi-arm comparisons on a single experimental block. And with custom conjugation, researchers are now able to customize with their preferred protein targets.”
Features and enhancements in the new kit include:
- High-throughput for running 64 samples in one experiment block in one week
- Custom conjugation allows researchers to conjugate their own validated antibodies to the platform’s barcoded oligos, adding targets from any indication area, including neuroscience, stem cell biology, infectious disease, and oncology, to the architectural readout. Up to two custom protein targets per reaction are included. Any surface protein with a working antibody can now participate in a Cell Surface Architecture experiment.
- Ease of use: Running 8,000 cells/8 samples in one reaction ensures easier processing and fewer batch effect.
- Sample input decreases cell input number from 500,000 in v1 to 50,000 cells/sample in v2
- Software allows 8000 cells per analysis processing runs
- Scalability and pricing: 3 kit configurations (8/16/32 reactions) with volume-based discounts available
The Pixelgen Proxiome Kit v2 runs on the Proximity Network Assay, a protein interactomics engine that uses barcoded antibodies, in situ rolling circle amplification, and standard DNA sequencing to produce a three-dimensional map of over 40,000 molecular nodes per cell and roughly 12,000 pairwise colocalization relationships across 1,000 single cells per sample. Every Proxiome Kit runs on standard Illumina sequencing infrastructure, requires no specialist imaging hardware, and is compatible with the PFA-fixation protocols most labs already use. Data analysis runs through Pixelator, the open-source pipeline available on nf-core. Samples from PBMCs, bone marrow, cell lines, and dissociated organoids are all supported.
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About Pixelgen TechnologiesÂ
Pixelgen Technologies AB is on a mission to deliver biology at the highest resolution by understanding the molecular basis of cellular function, with new tools and technologies that decipher the cell surface architecture of single cells in unprecedented ways. The company’s patented Proximity Network Assay delivers nanoscale spatial analysis of immune cell proteins at ultra-high capacity and underpins the Pixelgen Proxiome Kit for precision medicine. A foundational technology, the Proximity Network Assay will power a portfolio of products for translational research to accelerate advances in immunology, hematology, and cell therapy. Pixelgen was founded in 2020 by a team of passionate, experienced innovators and entrepreneurs and is venture-backed.
Contacts
Corporate:
Annika Branting
annika.branting@pixelgen.com
+46 762-69 68 46
Media:
Susan Thomas
susan@endpointcommunications.net
+1 (619) 540-9195